Phage Display: Methods and Protocols

Preface...Table of Contents...Contributing Authors...
Part I Construction of Antibody Phage Display Libraries
1. Construction of Human Immune and Naïve scFv LibrariesJonas Kügler, Florian Tomszak, André Frenzel, and Michael Hust
2. Construction of Naïve and Immune Human Fab Phage Display LibraryNoorsharmimi Omar and Theam Soon Lim
3. Construction of Synthetic Antibody Phage Display LibrariesJohan Nilvebrant and Sachdev S. Sidhu
4. Modular Construction of Large Non-Immune Human Antibody Phage Display Libraries from Variable Heavy and Light Chain Gene CassettesNam-Kyung Lee, Scott Bidlingmaier, Yang Su, and Bin Liu
5. Construction of Macaque Immune LibrariesArnaud Avril, Sebastian Miethe, Michael Hust, and Thibaut Pelat
6. Construction of Bovine Immunoglobulin Libraries in the single chain Fragment variable (scFv) FormatUlrike S. Diesterbeck
7. Construction of Rabbit Immune Antibody LibrariesThi Thu Ha Nguyen, Jong Seo Lee, and Hyunbo Shim
8. Generation of Semi-Synthetic Shark IgNAR Single Domain Antibody LibrariesJulius Grzeschik, Doreen Könning, Simon Krah, Christian Schröter, Martin Empting, Harald Kolmar, and Stefan Zielonka
9. Construction of High-Quality Camel Immune Antibody LibrariesEma Romão, Vianney Poignavent, Cécile Vincke, Christophe Ritzenthaler, Serge Muyldermans, and Baptiste Monsion
10. Construction of Chicken Antibody LibrariesJeanni Fehrsen, Susan Wemmer, and Wouter van Wyngaardt
11. Construction and Selection of Affilin Phage Display LibrariesFlorian Settele, Madlen Zwarg, Sebastian Fiedler, Daniel Koscheinz, and Eva Bosse-Doenecke
12. Construction of a Synthetic Antibody Gene Library for the Selection of Intrabodies and AntibodiesDéborah Caucheteur, Gautier Robin, Vincent Parez, and Pierre Martineau
13. Targeting Intracellular Antigens with pMHC-Binding Antibodies: A Phage Display ApproachZhihao Wu, Brian H. Santich, Hong Liu, Cheng Liu, and Nai-Kong V. Cheung
Part II Selection Strategies for Antibodies
14. Parallelized Antibody Selection in Microtiter PlatesGiulio Russo, Doris Meier, Saskia Helmsing, Esther Wenzel, Fabian Oberle, André Frenzel, and Michael Hust
15. Mass Spectrometry Immuno Assay (MSIA (TM)) Streptavidin Disposable Automation Research Tips (D.A.R.T's(R)) Antibody Phage Display BiopanningChai Fung Chin, Yee Siew Choong, and Theam Son Lim
16. Magnetic Nanoparticle-Based Semi-Automated Panning for High-Throughput Antibody SelectionAngela Chiew Wen Ch'ng, Nurul Hamizah binti Hamidon, Zoltán Konthur, and Thean Soon Lim
17. Phage Display and Selections on CellsWieland Fahr and André Frenzel
18. Combine Phage Antibody Display Library Selection on Patient Tissue Specimens with Laser Capture Microdissection to Identify Novel Human Antibodies Targeting Clinically Relevant Tumor AntigensYang Su, Scott Bidlingmaier, Nam-Kyung Lee, and Bin Liu
19. Antibody Isolation Form a Human Synthetic Combinational and Other Libraries of Single-Chain AntibodiesAlmog Bitton, Limor Nahary, and Itai Benhar
20. Screening Phage Display Antibody Libraries using Protein arraysRicardo Jara-Acevedo, Paula Díez, María González-González, Rosa María Dégano, Nieves Ibarrola, Rafael Góngora, Alberto Orfao, an