Crispr-Cas Methods
暫譯: Crispr-Cas 方法

本卷詳細介紹了 CRISPR-Cas 系統 的基本原理，其協議展示了 CRISPR-Cas 技術在高效基因組編輯方面的進展。入門章節提供了廣泛的 CRISPR/Cas 基礎方法 和應用視野。後續章節引導讀者了解 HDR 介導的編輯、sgRNA 設計、在水稻中進行多重腺嘌呤碱基編輯實驗的逐步程序、為水稻、小麥、短穗禾、麥芽、大麻、和疫霉生成突變體、突變體的視覺篩選、基因刪除（knock-out）、在哺乳動物細胞中的標記（knock-in）、無克隆（DNA-free）技術、細胞穿透肽、生成基因組編輯的香蕉，以及利用 CRISPR-Cpf1 結合單鏈 DNA（ssODN）修復模板進行的綠藻核基因組編輯。
《CRISPR-Cas 方法》權威且前沿，旨在協助對該領域不熟悉的研究人員，幫助他們學習如何最佳地將這項技術應用於特定生物體。

​M. Tofazzal Islam is a Professor and Director of the Institute of Biotechnology and Genetic Engineering (IBGE) of Bangabandhu Sheikh Mujibur Rahman Agricultural University in Bangladesh. He did his MS and Ph D in Applied Biosciences at Hokkaido University in Japan. Dr. Islam received postdoctoral research experiences at Hokkaido University, University of Göttingen, University of Nottingham and West Virginia University under the JSPS, Alexander von Humboldt, Commonwealth, and Fulbright Fellowships, respectively. He published articles in many international journals and book series (>200 peer-reviewed articles, total citation 2,550, h index 26, i10-index 69; RG score 40.72). Dr. Islam was awarded many prizes and medals including Bangladesh Academy of Science Gold Medal in 2011, University Grants Commission Bangladesh Awards in 2004 and 2008, and Best Young Scientist Award 2003 from the JSBBA. Prof. Islam is the Chief Editor of a book series, Bacillus and Agrobiotechnology publishes by Springer. He is an elected Fellow of Bangladesh Academy of Science. His research interests include genomics, genome editing, plant probiotics and novel biologicals, and bioactive natural products.
Dr. Pankaj Bhowmik graduated with honors from Bangladesh Agricultural University and joined at the Bangladesh Agricultural Research Institute as a Scientific Officer. He received his Ph.D. in Plant Physiology and Molecular Biology from Kagawa University, Japan. Following JSPS, Killam and NSERC post-doctoral training at the National Agricultural Research Center in Hokkaido, University of Alberta and Agriculture and Agri-Food Canada, Dr. Bhowmik joined the National Research Council of Canada in Saskatoon, where he is now a Research Officer and a member of the Cell Technologies team. Dr. Bhowmik with over 15 years of experience specializes in plant physiology, metabolomics, tissue culture, plant transformation, and gene editing. Since 2009 after joining NRC, he has been working on mesophyll protoplast and microspore culture, microspore transformation and plant regeneration of canola and wheat combining doubled haploidy and genetic engineering tools. Dr. Bhowmik is in the process of developing a crop-agnostic CRISPR based gene editing platform that can be deployed for the improvement of any high-value crops such as cereals, oilseeds, pulse crops and greenhouse vegetables. He is the project lead for the NRC's pulse quality improvement project under the Ag-Food initiative supporting Protein Industry Canada innovation supercluster.
Kutubuddin A. Molla is a faculty scientist in agricultural biotechnology at the ICAR-National Rice Research Institute, Cuttack, India. He undertook his university studies at the University of Calcutta, Kolkata, India, and holds MSc and Ph.D. degrees in Botany. He is a recipient of the prestigious Fulbright fellowship. Dr. Molla has done his post-doctoral research at the Pennsylvania State University, USA, for two years. Dr. Molla is interested in precise genome editing in crop plants and uses CRISPR/Cas-base editors and 'homology-directed repair' (HDR) methods for crop improvement. Dr. Molla is an expert in plant genetic engineering, transgenic technology, and genome editing. Dr. Molla bagged many prestigious awards, including the Young Scientist Award by Indian Science Congress Association, Jawaharlal Nehru best Ph.D. thesis award by Indian Council of Agricultural Research, Early Career Research Award by Department of Science and Technology, Govt. of India. He is also the recipient of the AAAS/Science Program for Excellence in Science from the American Association for the Advancement of Science (AAAS), Washington D.C. Dr. Molla published his research in many international peer-reviewed journals, including Trends in Biotechnology, Molecular Plant Pathology, Theoretical and Applied Genetics, Plant Science, BMC Plant Biology, Scientific Reports, GM crops & food, and Environmental and Experimental Botany.

M. Tofazzal Islam教授是孟加拉國邦甘德大學農業大學生物技術與基因工程研究所（IBGE）的所長。他在日本北海道大學獲得應用生物科學的碩士和博士學位。Islam博士曾在北海道大學、哥廷根大學、諾丁漢大學和西維吉尼亞大學獲得JSPS、亞歷山大·馮·洪堡、英聯邦和富布賴特獎學金的博士後研究經驗。他在許多國際期刊和書籍系列上發表了超過200篇的同行評審文章，總引用次數為2,550次，h指數為26，i10指數為69；RG分數為40.72。Islam博士獲得了許多獎項和獎牌，包括2011年孟加拉科學院金獎、2004年和2008年孟加拉大學獎學金委員會獎，以及2003年JSBBA的最佳青年科學家獎。Islam教授是由Springer出版的書籍系列《Bacillus and Agrobiotechnology》的主編。他是孟加拉科學院的當選院士。他的研究興趣包括基因組學、基因組編輯、植物益生菌和新型生物製劑，以及生物活性天然產品。

Pankaj Bhowmik博士以優異成績畢業於孟加拉農業大學，並在孟加拉農業研究所擔任科學官。他在日本香川大學獲得植物生理學和分子生物學的博士學位。隨後，他在北海道國立農業研究中心、阿爾伯塔大學和加拿大農業與農產品部接受了JSPS、Killam和NSERC的博士後培訓。Bhowmik博士目前在加拿大國家研究委員會的薩斯卡通工作，擔任研究官並是細胞技術團隊的成員。Bhowmik博士擁有超過15年的經驗，專注於植物生理學、代謝組學、組織培養、植物轉化和基因編輯。自2009年加入NRC以來，他一直在研究葉肉原生質體和小孢子培養、小孢子轉化以及結合雙倍單倍體和基因工程工具的油菜和小麥的植物再生。Bhowmik博士正在開發一個作物無關的基於CRISPR的基因編輯平台，該平台可用於改進任何高價值作物，如穀物、油籽、豆類作物和溫室蔬菜。他是NRC脈衝質量改進項目的項目負責人，該項目在支持加拿大蛋白質產業創新超集群的Ag-Food倡議下進行。

Kutubuddin A. Molla是印度卡塔克ICAR國立水稻研究所的農業生物技術教職科學家。他在印度加爾各答的加爾各答大學完成了大學學業，並獲得植物學的碩士和博士學位。他是著名的富布賴特獎學金獲得者。Molla博士在美國賓夕法尼亞州立大學進行了兩年的博士後研究。Molla博士對作物植物的精確基因組編輯感興趣，並使用基於CRISPR/Cas的編輯器和“同源重組修復”（HDR）方法進行作物改良。Molla博士是植物基因工程、轉基因技術和基因組編輯的專家。他獲得了許多著名獎項，包括印度科學大會的青年科學家獎、印度農業研究委員會的Jawaharlal Nehru最佳博士論文獎、印度政府科學與技術部的早期職業研究獎。他還是美國科學促進會（AAAS）頒發的科學卓越獎的獲得者。Molla博士在許多國際同行評審期刊上發表了他的研究，包括《生物技術趨勢》、《分子植物病理學》、《理論與應用遺傳學》、《植物科學》、《BMC植物生物學》、《科學報告》、《GM作物與食品》和《環境與實驗植物學》。