Rift Valley Fever Virus: Methods and Protocols

Lozach, Pierre-Yves

  • 出版商: Humana Press
  • 出版日期: 2024-07-23
  • 售價: $7,750
  • 貴賓價: 9.5$7,363
  • 語言: 英文
  • 頁數: 552
  • 裝訂: Hardcover - also called cloth, retail trade, or trade
  • ISBN: 1071639250
  • ISBN-13: 9781071639252
  • 下單後立即進貨 (約1週~2週)

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商品描述

This volume looks at the latest techniques used in the study of Rift Valley Fever Virus (RVFV). The chapters in this book cover topics such as molecular biology, biochemistry, and cell biology approaches that enable the mechanistic study of virus-host interactions; vector competence, detection, and diagnosis; virus inactivation for biosafety purposes; virus and protein production for structural studies; high-throughput screening based on CRISPR-Cas9 technology; single-molecule RNA imaging; and physiological infection models, animal models, and immunology. Written in the high successful Methods in Molecular Biology format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Rift Valley Fever Virus: Methods and Protocols is a valuable resource for both novice and experts researchers who want to learn more about the important and developing field of RVFV.

商品描述(中文翻譯)

本書探討了在裂谷熱病毒(RVFV)研究中使用的最新技術。本書的章節涵蓋了分子生物學、生物化學和細胞生物學等方法,這些方法使得能夠對病毒與宿主之間的機制進行研究;蚊媒的能力、檢測和診斷;病毒灭活以用於生物安全;病毒和蛋白質的生產以進行結構研究;基於CRISPR-Cas9技術的高通量篩選;單分子RNA成像;以及生理感染模型、動物模型和免疫學。本書以成功的《分子生物學方法》格式撰寫,章節包括對各自主題的介紹、所需材料和試劑的清單、逐步可重複的實驗室協議,以及疑難解答和避免已知問題的提示。《裂谷熱病毒:方法與協議》是一本尖端且全面的資源,對於想要更多了解RVFV這一重要且發展中領域的初學者和專家研究人員都非常有價值。